4G0B
Structure of native HCT from Coffea canephora
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-05 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.873 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 116.100, 116.100, 158.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.1893 |
Rwork | 0.186 |
R-free | 0.25004 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bgh |
RMSD bond length | 0.011 |
RMSD bond angle | 1.560 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.166 | 0.566 |
Number of reflections | 22073 | |
<I/σ(I)> | 8.5 | 3.1 |
Completeness [%] | 99.0 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M magnesium sulfate and 0.1 M MES, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.5 |