4G04
High-resolution Crystal Structural Variance Analysis between Recombinant and Wild-type Human Serum Albumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 58.745, 58.664, 95.726 |
Unit cell angles | 87.85, 75.92, 73.47 |
Refinement procedure
Resolution | 47.365 - 2.301 |
R-factor | 0.2431 |
Rwork | 0.240 |
R-free | 0.31660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ao6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.159 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.365 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.095 | |
Number of reflections | 52509 | |
<I/σ(I)> | 22.48 | |
Completeness [%] | 90.6 | 90.6 |
Redundancy | 34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 28-30% (w/v), PEG3350, Sigma-Aldrich), 50mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, temperature 291K |