4FZZ

Exonuclease X in complex with 5' overhanging duplex DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	37.192, 79.009, 156.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.010 - 2.800
R-factor	0.2253
Rwork	0.223
R-free	0.26009
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fzx
RMSD bond length	0.005
RMSD bond angle	0.688
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.960
High resolution limit [Å]	2.800	2.800
Number of reflections	11031
Completeness [%]	93.5	93.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	289	0.1M MES, 17% PEG6000, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K