4FZN
Crystal structure of syringacin M mutant D232A from Pseudomonas syringae pv. tomato DC3000
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 160.350, 160.350, 100.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.090 - 3.120 |
R-factor | 0.18417 |
Rwork | 0.182 |
R-free | 0.22359 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fzm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.513 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.090 | 3.200 |
High resolution limit [Å] | 3.120 | 3.120 |
Rmerge | 0.048 | 0.787 |
Number of reflections | 13347 | |
<I/σ(I)> | 26.9 | 4.3 |
Completeness [%] | 99.5 | 100 |
Redundancy | 15.5 | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 7% w/v PEG 8000, 30% v/v ethylene glycol 0.03 M CaCl2, 0.03 M MgCl2, 5% dimethyl sulfoxide, 0.1 M Bicine/Tris base, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |