4FZN
Crystal structure of syringacin M mutant D232A from Pseudomonas syringae pv. tomato DC3000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 160.350, 160.350, 100.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.090 - 3.120 |
| R-factor | 0.18417 |
| Rwork | 0.182 |
| R-free | 0.22359 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fzm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.090 | 3.200 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.048 | 0.787 |
| Number of reflections | 13347 | |
| <I/σ(I)> | 26.9 | 4.3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 15.5 | 16.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 7% w/v PEG 8000, 30% v/v ethylene glycol 0.03 M CaCl2, 0.03 M MgCl2, 5% dimethyl sulfoxide, 0.1 M Bicine/Tris base, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
