4FZM
Crystal structure of the bacteriocin syringacin M from Pseudomonas syringae pv. tomato DC3000
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795, 0.9796, 0.9253 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 159.687, 159.687, 100.173 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.340 - 2.830 |
R-factor | 0.20227 |
Rwork | 0.200 |
R-free | 0.23914 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 2.028 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.340 | 2.910 |
High resolution limit [Å] | 2.830 | 2.830 |
Rmerge | 0.059 | 0.693 |
Number of reflections | 17330 | |
<I/σ(I)> | 19.5 | 3.1 |
Completeness [%] | 99.1 | 100 |
Redundancy | 8.2 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 7% w/v PEG 8000, 30% v/v ethylene glycol 0.03 M CaCl2, 0.03 M MgCl2, 5% dimethyl sulfoxide, 0.1 M Bicine/Tris base, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |