4FYU

Crystal structure of Thioredoxin from Wuchereria bancrofti at 2.0 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Wavelength(s)	0.8123
Spacegroup name	I 4 2 2
Unit cell lengths	112.610, 112.610, 162.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.250 - 2.000
R-factor	0.173
Rwork	0.170
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	2.015
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.940
Number of reflections	35142
Completeness [%]	97.0
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	100mM Tris HCL pH 5.6, 200mM NaCl, 24% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K