4FYJ

Crystal Structure of P. aeruginosa peptidyl-tRNA hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-01-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97
Spacegroup name	P 61 2 2
Unit cell lengths	63.619, 63.619, 155.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.809 - 1.770
R-factor	0.193
Rwork	0.191
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pth
RMSD bond length	0.007
RMSD bond angle	0.673
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.770	3.810	1.770
Rmerge	0.045	0.022	0.258
Number of reflections	18791
<I/σ(I)>	17.1
Completeness [%]	99.8	98.5	100
Redundancy	11	10.1	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		PEG 4000 isopropanol, pH 7.5, sitting-drop vapor diffusion, temperature 100K