4FYH
Crystal structure of rcl with phospho-triciribine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9334 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.953, 119.002, 57.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.500 - 2.440 |
R-factor | 0.21093 |
Rwork | 0.209 |
R-free | 0.24645 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.093 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.570 | |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.376 | |
<I/σ(I)> | 5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 100 mM Tris, 1.2 Li2SO4, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.K |