4FXP
Crystal structure of adenosine 5'-phosphosulfate kinase from Arabidopsis thaliana in Complex with Sulfate and APS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.948, 92.348, 73.183 |
| Unit cell angles | 90.00, 113.52, 90.00 |
Refinement procedure
| Resolution | 33.857 - 1.954 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.900 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 52723 |
| Completeness [%] | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 277 | 0.1 M hepes, 200 mM magnesium chloride, 15 % PEG 2000, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
