4FXL
Crystal structure of the D76N Beta-2 Microglobulin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-09-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.220, 28.420, 52.260 |
| Unit cell angles | 90.00, 126.54, 90.00 |
Refinement procedure
| Resolution | 18.800 - 1.400 |
| R-factor | 0.13275 |
| Rwork | 0.130 |
| R-free | 0.18616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lds |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.048 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.800 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.375 |
| Number of reflections | 17632 | |
| <I/σ(I)> | 10.1 | 2.9 |
| Completeness [%] | 98.4 | 98.9 |
| Redundancy | 3.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 18-20% PEG 4000, 20% glycerol, 0.2M ammonium acetate, 0.1 M MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
