4FXD
Crystal structure of yeast DNA polymerase alpha bound to DNA/RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.140, 74.770, 116.990 |
| Unit cell angles | 82.26, 72.57, 82.40 |
Refinement procedure
| Resolution | 55.526 - 3.000 |
| R-factor | 0.2555 |
| Rwork | 0.254 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvm |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.766 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1078)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.770 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.073 | 0.921 |
| Number of reflections | 45027 | |
| <I/σ(I)> | 13.6 | 1.5 |
| Completeness [%] | 97.8 | 97.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M Bicine, 12% PEG3350, 10 mM MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
