4FX0
Crystal structure of M. tuberculosis transcriptional regulator MosR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-03-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.998, 66.677, 79.089 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.846 - 2.698 |
R-factor | 0.2326 |
Rwork | 0.231 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.579 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 2.770 |
High resolution limit [Å] | 2.698 | 2.698 |
Number of reflections | 7602 | |
Completeness [%] | 95.5 | 78.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.2 M SODIUM CITRATE, PH 5.5, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |