4FWI
Crystal structure of the nucleotide-binding domain of a dipeptide ABC transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 64.247, 64.247, 320.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.809 - 2.892 |
R-factor | 0.2146 |
Rwork | 0.212 |
R-free | 0.26790 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.477 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.920 | 2.990 |
High resolution limit [Å] | 2.892 | 3.640 | 2.890 |
Number of reflections | 15715 | ||
Completeness [%] | 97.5 | 98.4 | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 11 | 291 | 4%(v/v) 2-propanol, 0.05M capso, 12.25%(w/v) PEG MME5000, 12.5% glycerol, pH 11.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |