4FWE
Native structure of LSD2 /AOF1/KDM1b in spacegroup of C2221 at 2.13A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2012-04-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97943 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.691, 96.273, 182.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.130 |
| R-factor | 0.20486 |
| Rwork | 0.203 |
| R-free | 0.24556 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fwj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.359 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.889 | |
| Number of reflections | 44518 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 93.9 | 85.6 |
| Redundancy | 10.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 289 | 21% PEG3350, 200mM diammonium citrate, pH 7, EVAPORATION, temperature 289K |
