4FVS
Crystal structure of a putative lipoprotein (BDI_3050) from Parabacteroides distasonis ATCC 8503 at 2.70 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9785,0.9792,0.91162 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.860, 154.510, 72.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.700 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22430 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SOLVE |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.830 | 29.830 | 2.770 |
| High resolution limit [Å] | 2.700 | 12.070 | 2.700 |
| Rmerge | 0.036 | 0.664 | |
| Total number of observations | 1697 | 11871 | |
| Number of reflections | 44235 | ||
| <I/σ(I)> | 10.6 | 17.9 | 1.1 |
| Completeness [%] | 99.8 | 91.5 | 99.9 |
| Redundancy | 3.7 | 3.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 20.0% polyethylene glycol 8000, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
