4FVM
Crystal structure of yeast DNA polymerase alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9754 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.380, 127.140, 74.570 |
| Unit cell angles | 90.00, 104.78, 90.00 |
Refinement procedure
| Resolution | 36.512 - 2.300 |
| R-factor | 0.2068 |
| Rwork | 0.205 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b08 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.586 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1078)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.510 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.512 |
| Number of reflections | 58906 | |
| <I/σ(I)> | 11.3 | 3 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 291 | 0.1M Bicine, 6% PEG8000, pH 9.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
