4FVJ
SPFH domain of the mouse stomatin (Crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.918410 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.530, 91.380, 86.138 |
Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
Resolution | 82.760 - 2.690 |
R-factor | 0.24783 |
Rwork | 0.246 |
R-free | 0.29009 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fvf |
RMSD bond length | 0.005 |
RMSD bond angle | 0.920 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.760 | 2.740 |
High resolution limit [Å] | 2.670 | 2.690 |
Number of reflections | 32523 | |
<I/σ(I)> | 6.8 | 2.7 |
Completeness [%] | 91.3 | 76.2 |
Redundancy | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 7% ethanol, 5% glycerol, 0.8 M sodium acetate, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |