4FVF
SPFH domain of mouse stomatin (Crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-11-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.500, 55.500, 343.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.260 - 2.460 |
| R-factor | 0.21975 |
| Rwork | 0.217 |
| R-free | 0.26849 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rpb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.954 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.260 | 2.610 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.075 | 1.851 |
| Number of reflections | 12351 | |
| <I/σ(I)> | 22.67 | 4.12 |
| Completeness [%] | 99.3 | 0.958 |
| Redundancy | 10.8 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1 M sodium acetate, 0.05 M cadmium sulfate, 0.1 M HEPES , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
