4FVC
HmoB structure with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Collection date | 2012-05-10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.821, 117.519, 70.847 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 19.580 - 1.750 |
| R-factor | 0.20056 |
| Rwork | 0.199 |
| R-free | 0.23257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 112141 | |
| Completeness [%] | 95.8 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.5% PEG2000, 1.2M Na-Citrate, 0.1M HEPES pH7.0, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
