4FVB
Crystal structure of EV71 2A proteinase C110A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.613, 44.265, 52.036 |
| Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hrv |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.024 |
| Data reduction software | AUTOMAR |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.035 | 0.262 |
| Number of reflections | 13431 | |
| <I/σ(I)> | 11.9 | 2.1 |
| Completeness [%] | 92.4 | 96 |
| Redundancy | 1.93 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 100mM HEPES, 20% 2-propanol, 10% PEG 4000, pH 7.5, vapor diffusion, hanging drop, temperature 289K |
