4FV3
Crystal Structure of the ERK2 complexed with EK6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.780, 70.700, 60.860 |
| Unit cell angles | 90.00, 110.11, 90.00 |
Refinement procedure
| Resolution | 30.930 - 2.200 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 120.000 | 120.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 5.040 | 2.200 |
| Rmerge | 0.050 | 0.029 | 0.221 |
| Number of reflections | 19388 | ||
| <I/σ(I)> | 17 | ||
| Completeness [%] | 97.8 | 90.7 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES buffer, pH 6.5, 26-28% PEG-MME 2000, 200 mM ammonium sulfate and 20 mM 2-mercaptoethanol, vapor diffusion, temperature 298K |
