4FUT
Crystal structure of ATP bound MatB from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 173.140, 173.140, 47.739 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.991 - 2.000 |
| R-factor | 0.1598 |
| Rwork | 0.158 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apo-rpMatb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.454 |
| <I/σ(I)> | 47.867 | 7.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.3 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | (Bis-Tris buffer (100 mM, pH 6.5) containing trimethylammonium chloride (25 mM), polyethylene glycol 8000 (19.5 %, w/v), glycerol (5 %, w/v), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
