4FUS
The X-ray structure of Hahella chejuensis family 48 glycosyl hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-11 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.690, 78.389, 67.075 |
Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.750 |
R-factor | 0.15639 |
Rwork | 0.154 |
R-free | 0.20466 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9g |
RMSD bond length | 0.021 |
RMSD bond angle | 2.069 |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 63636 | |
<I/σ(I)> | 12.71 | 1.93 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.47 | 3.41 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 0.05 M potassium phosphate monobasic and 20 % (w/v) polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |