4FUP
Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.101, 34.240, 138.779 |
| Unit cell angles | 90.00, 101.31, 90.00 |
Refinement procedure
| Resolution | 45.360 - 2.510 |
| R-factor | 0.2219 |
| Rwork | 0.219 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.078 | 0.057 | 0.200 |
| Number of reflections | 13488 | ||
| <I/σ(I)> | 16.5 | ||
| Completeness [%] | 99.4 | 97.5 | 99.7 |
| Redundancy | 3.6 | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.9 | 293 | 0.12 M Postassium Thiocyanate, 0.1 M Tris pH7.9, 28% PEG MME 2000, 0.5% beta-octylglucopyranoside, batch, temperature 293K |
