4FUL
PI3 Kinase Gamma bound to a pyrmidine inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.040, 66.900, 102.820 |
Unit cell angles | 90.00, 97.59, 90.00 |
Refinement procedure
Resolution | 33.340 - 2.470 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.900 | 2.530 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.064 | 0.638 |
Number of reflections | 34136 | |
<I/σ(I)> | 13.7 | 2.2 |
Completeness [%] | 99.5 | 99.7 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 100 mM tris HCl pH8.3, 100 mM Ammonium Sulfate, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |