4FTL
Crystal Structure of the CHK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.481, 65.772, 58.229 |
Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
Resolution | 19.740 - 2.500 |
R-factor | 0.1806 |
Rwork | 0.178 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.950 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.350 | 2.500 |
Rmerge | 0.065 | 0.044 | 0.239 |
Number of reflections | 11875 | ||
<I/σ(I)> | 17.9 | ||
Completeness [%] | 99.4 | 99.3 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 8000, Isopropanol, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |