4FTH
Crystal Structure of NtrC4 DNA-binding domain bound to double-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-04-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.436, 28.798, 74.931 |
Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
Resolution | 48.108 - 3.004 |
R-factor | 0.2452 |
Rwork | 0.243 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3e7l |
RMSD bond length | 0.004 |
RMSD bond angle | 0.802 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.063 | |
Number of reflections | 5777 | |
<I/σ(I)> | 15.5 | |
Completeness [%] | 96.8 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 30 mM Na-cacodylate pH 6.0, 140 mM spermidine, 50 mM potassium thiocyanate, and 15% (poly)ethylene glycol mw 400, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |