4FSK
Urate oxidase-azide complex in anaerobic conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.982 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.858, 95.338, 104.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.230 - 1.980 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3p9f |
| RMSD bond length | 0.034 |
| RMSD bond angle | 0.077 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.230 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.054 | 0.195 |
| Number of reflections | 26720 | |
| <I/σ(I)> | 3.4 | 19.5 |
| Completeness [%] | 97.8 | 93.9 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | 291 | PROTEIN AT 20 MG/ML IN 50 MM TRIS BUFFER, 0.3 M SODIUM AZIDE, SATURATED WITH URIC ACID, pH 10.0, BATCH METHOD - STTING DROPS, temperature 291K |
