4FS5
Crystal structure of the Z-DNA hexamer CGCGCG at 500 mM MgCl2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 18.593, 18.593, 72.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.682 - 1.300 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.26350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1io5 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.849 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.200 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.054 | 0.120 |
Number of reflections | 6868 | |
Completeness [%] | 99.2 | 99 |
Redundancy | 6.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | TEMPERATURE CONTROL | 7 | 293 | 2.0mM DNA, 20mM Na cacodylate, 30% 2-methyl-2,4-pentainediol, 500mM MgCl2, pH 7.0, TEMPERATURE CONTROL, temperature 293K |