4FS0
Crystal structure of mutant F136D of mouse nectin-2 extracellular fragment D1-D2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-03 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 59.857, 59.857, 210.013 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.250 |
R-factor | 0.24979 |
Rwork | 0.240 |
R-free | 0.33476 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fmk |
RMSD bond length | 0.012 |
RMSD bond angle | 1.556 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.370 |
High resolution limit [Å] | 3.250 | 6.980 | 3.250 |
Number of reflections | 19387 | ||
<I/σ(I)> | 13.7 | 2.5 | |
Completeness [%] | 99.8 | 99.6 | 93.2 |
Redundancy | 6.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 1 M lithium sulfate, 0.6 M ammonium sulfate, 0.1 M tri-sodium citrate, pH 5.5, cryoprotectant: 30% w/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |