4FRX
Crystal Structure of Pseudomonas aeruginosa OccK8 (OprE)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.10 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.386, 90.121, 103.598 |
Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
Resolution | 14.993 - 1.900 |
R-factor | 0.214 |
Rwork | 0.213 |
R-free | 0.24690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sys |
RMSD bond length | 0.007 |
RMSD bond angle | 1.137 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 99602 | |
<I/σ(I)> | 9.2 | 1.93 |
Completeness [%] | 99.3 | 98.6 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% PEG 400, 0.2M ammonium sulphate, 0.05M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |