4FRU
Crystal structure of horse wild-type cyclophilin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.827 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.880, 44.070, 60.570 |
| Unit cell angles | 90.00, 95.20, 90.00 |
Refinement procedure
| Resolution | 16.416 - 1.100 |
| R-factor | 0.1167 |
| Rwork | 0.116 |
| R-free | 0.13910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cyn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.301 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.420 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.078 | 0.272 |
| Number of reflections | 62397 | |
| <I/σ(I)> | 9.8 | 3.9 |
| Completeness [%] | 90.4 | 53.4 |
| Redundancy | 3.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295 | 0.1M MES, 10mM ZnCl2, 10% glycerol, 28% PEG MME 550, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
