4FRT
Crystal Structure of Pseudomonas aeruginosa OccK7 (OpdD)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9764 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.250, 111.154, 99.933 |
Unit cell angles | 90.00, 123.25, 90.00 |
Refinement procedure
Resolution | 14.989 - 3.166 |
R-factor | 0.2127 |
Rwork | 0.210 |
R-free | 0.26870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t24 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.395 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 23348 | |
<I/σ(I)> | 18 | 3.55 |
Completeness [%] | 99.1 | 98.8 |
Redundancy | 7.7 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 400, 1M sodium formate, 0.05M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |