4FQT
Structure of AgamOBP1 Bound to 6-methyl-5-hepten-2-one
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2010-06-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.769, 68.993, 64.552 |
Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
Resolution | 31.697 - 2.200 |
R-factor | 0.1877 |
Rwork | 0.185 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.413 |
Data reduction software | d*TREK (9.7L) |
Data scaling software | d*TREK (9.7L) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.260 | 46.260 | 1.630 |
High resolution limit [Å] | 1.570 | 3.380 | 1.570 |
Rmerge | 0.151 | 0.074 | 0.750 |
Number of reflections | 26439 | ||
<I/σ(I)> | 4.5 | 11.4 | 0.3 |
Completeness [%] | 68.4 | 99.3 | 3.8 |
Redundancy | 3.17 | 3.74 | 1.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |