4FQQ
Crystal Structure of Germline Antibody PGT121-GL Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.934, 344.743, 55.230 |
| Unit cell angles | 90.00, 91.95, 90.00 |
Refinement procedure
| Resolution | 37.440 - 2.420 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.951 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.305 | 38.305 | 2.550 |
| High resolution limit [Å] | 2.419 | 7.650 | 2.420 |
| Rmerge | 0.028 | 0.482 | |
| Number of reflections | 74318 | ||
| <I/σ(I)> | 11 | 22 | 1.6 |
| Completeness [%] | 95.5 | 96.2 | 80.1 |
| Redundancy | 3.1 | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | PEG 3350, sodium malonate, MnCl2, pH 7.0, vapor diffusion, temperature 298K |
