4FQP
Crystal structure of human Nectin-like 5 full ectodomain (D1-D3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-30 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 138.919, 138.919, 273.645 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.600 |
R-factor | 0.24953 |
Rwork | 0.248 |
R-free | 0.27248 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3EOW |
RMSD bond length | 0.013 |
RMSD bond angle | 1.761 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.730 |
High resolution limit [Å] | 3.563 | 3.563 |
Number of reflections | 19387 | |
<I/σ(I)> | 13.7 | 2.5 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293.15 | 55% v/v tacsimate, 0.1 M Bicine, pH 9.0, with additional 10% tacsimate as cryoprotectant, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |