4FQE
KdgM porin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.008 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.856, 59.674, 51.564 |
Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.930 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wjq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.503 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (CCP4_3.3.16 2010/01/06) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.360 | 49.360 | 2.030 |
High resolution limit [Å] | 1.930 | 6.100 | 1.930 |
Rmerge | 0.073 | 0.030 | 0.390 |
Total number of observations | 1933 | 8662 | |
Number of reflections | 16187 | ||
<I/σ(I)> | 10.4549 | 18.76 | 1.57 |
Completeness [%] | 99.9 | 98.65 | 99.93 |
Redundancy | 3.78 | 3.64 | 3.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES, 22% MPD, 36mM digalacturonate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |