4FQ1
Crystal Structure of PGT121 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-03 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.753, 74.672, 114.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.510 - 3.000 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.971 |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.306 | 2.930 |
High resolution limit [Å] | 2.776 | 2.780 |
Rmerge | 0.293 | |
Number of reflections | 12460 | |
<I/σ(I)> | 8.8 | 2.7 |
Completeness [%] | 96.7 | 84.8 |
Redundancy | 3.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 298 | PEG 4000, Tris HCl, CuCl2, pH 8.5, vapor diffusion, temperature 298K |