4FOP
Crystal Structure of Peptidyl-tRNA hydrolase from Acinetobacter baumannii at 1.86 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 34.255, 57.864, 109.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.800 - 1.860 |
| R-factor | 0.17785 |
| Rwork | 0.175 |
| R-free | 0.22795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pth |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.036 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.800 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 17986 | |
| <I/σ(I)> | 11.6 | 1.7 |
| Completeness [%] | 99.1 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 310 | 0.2M HEPES Buffer, 25% PEG10000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
