4FO8
Pseudomonas aeruginosa MetAP with Met, in Mn form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.651, 105.664, 133.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.770 - 1.900 |
R-factor | 0.1593 |
Rwork | 0.157 |
R-free | 0.20010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 1.930 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.920 |
High resolution limit [Å] | 1.900 | 6.500 | 1.900 |
Rmerge | 0.075 | 0.049 | 0.222 |
Number of reflections | 80038 | ||
<I/σ(I)> | 9.6 | ||
Completeness [%] | 88.6 | 83.4 | 67.7 |
Redundancy | 4 | 4.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion, Met soaked in | 7.5 | 100 mM HEPES, 70% MPD, pH 7.5, hanging drop vapor diffusion, Met soaked in |