4FMZ

Crystal structure of an internalin (inlF) from Listeria monocytogenes str. 4b F2365 at 1.91 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97929,0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	46.191, 102.548, 128.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.480 - 1.910
R-factor	0.1778
Rwork	0.176
R-free	0.20980
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.480		1.980
High resolution limit [Å]	1.910	4.100	1.910
Rmerge	0.070	0.024	0.485
Number of reflections	47630
<I/σ(I)>	8.7	26.5	1.7
Completeness [%]	96.2	96.1	96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.4M tri-Sodium Citrate, 0.1M sodium HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K