4FMU
Crystal structure of Methyltransferase domain of human SET domain-containing protein 2 Compound: Pr-SNF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-11 |
Detector | MAR 300 CCD |
Wavelength(s) | 1.03321 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.547, 77.277, 78.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.120 - 2.100 |
R-factor | 0.1954 |
Rwork | 0.194 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3H6L |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.059 | 0.221 |
Number of reflections | 18501 | |
<I/σ(I)> | 42.7 | 6.08 |
Completeness [%] | 97.0 | 82.3 |
Redundancy | 7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG4000, 10% isoprpanol, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |