4FMM
Dimeric Sec14 family homolog 3 from Saccharomyces cerevisiae presents some novel features of structure that lead to a surprising "dimer-monomer" state change induced by substrate binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.177, 76.914, 294.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.414 - 2.340 |
R-factor | 0.21696 |
Rwork | 0.214 |
R-free | 0.26363 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 147.480 | 2.380 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.057 | 0.105 |
Number of reflections | 37249 | |
Completeness [%] | 95.7 | 76 |
Redundancy | 6.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.0, 20% w/v PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |