4FMH
Merkel Cell Polyomavirus VP1 in complex with Disialyllactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-05-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.270, 85.770, 248.710 |
| Unit cell angles | 92.97, 100.48, 108.05 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.16595 |
| Rwork | 0.166 |
| R-free | 0.19575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.096 | 0.612 |
| Number of reflections | 529047 | |
| <I/σ(I)> | 9.52 | 1.74 |
| Completeness [%] | 94.7 | 90 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium cacodylate, pH 6.5, 6% w/v PEG3350, 0.3 M magnesium chloride, soaked in 20 mM disialyllactose, cryoprotection: 25% w/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
