4FMA
EspG structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.615, 167.739, 361.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.23880 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.145 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.065 | 0.027 | 0.412 |
Number of reflections | 310382 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 100.0 | 99.7 | 99.9 |
Redundancy | 7.5 | 7.2 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.5 M sodium formate, 0.1 M Bis-Tis propane (pH 7.0), and 0.2 M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |