4FLF
Structure of three phase partition treated lipase from Thermomyces lanuginosa at 2.15A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-05-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 61 |
Unit cell lengths | 139.996, 139.996, 80.503 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.22658 |
Rwork | 0.224 |
R-free | 0.26841 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ea6 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.018 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 46024 | |
<I/σ(I)> | 33 | 2.17 |
Completeness [%] | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 0.1M Nacl, 1.6M Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |