4FL9

Crystal Structure of bovine hsc70(aa1-554)E213A/D214A at 1.9A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	66.255, 50.380, 86.638
Unit cell angles	90.00, 99.65, 90.00

Refinement procedure

Resolution	38.850 - 1.901
R-factor	0.1941
Rwork	0.192
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge		0.597
Number of reflections	44250
<I/σ(I)>	12.2	1.84
Completeness [%]	98.9	95.5
Redundancy	3.46	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291	PEG8000,TRIMETHYL AMINE OXIDE , pH 7.5, Microbatch, temperature 291K