4FL1
Structural and Biophysical Characterization of the Syk Activation Switch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-11-26 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.083, 85.403, 92.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.400 - 1.790 |
R-factor | 0.197 |
Rwork | 0.192 |
R-free | 0.22460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.400 |
High resolution limit [Å] | 1.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M HEPES, 28% PEG 3350, 0.2 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |