4FKD
Identification of the Activator Binding Residues in the Second Cysteine-Rich Regulatory Domain of Protein Kinase C Theta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-11 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 61 |
| Unit cell lengths | 65.850, 65.850, 27.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.514 - 1.633 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (MR) |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.514 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.044 | 0.115 |
| Number of reflections | 7559 | |
| Completeness [%] | 88.4 | 98 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1 M Lithium acetate and 20% PEG 3350, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
