4FJU
Crystal structure of ureidoglycolate dehydrogenase in ternary complex with NADH and glyoxylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-05 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 162.756, 162.756, 61.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.393 - 1.771 |
R-factor | 0.2296 |
Rwork | 0.229 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3QGR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.141 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.074 | 0.583 |
Number of reflections | 74082 | |
<I/σ(I)> | 46.7 | 4.6 |
Completeness [%] | 99.7 | 97.3 |
Redundancy | 15.7 | 15.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 295 | 0.1M MES, 4.0M NACL, pH 6.0, EVAPORATION, temperature 295K |